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cwlS [2018-02-13T15:04:28.000Z]
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cwlS [2018-02-13T15:04:28.000Z]

D,L-endopeptidase, peptidoglycan hydrolase for cell separation
Locus
BSU19410
Isoelectric point
10.44
Molecular weight
44.07 kDa
Protein length
414 aa Sequence Blast
Gene length
1242 bp Sequence Blast
Function
cell separation
Product
D,L-endopeptidase, peptidoglycan hydrolase
Essential
no
Synonyms
yojL

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,115,425 → 2,116,669

The protein

Catalyzed reaction/ biological activity

  • cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) PubMed
  • Protein family

  • Cu-Zn superoxide dismutase family (according to Swiss-Prot)
  • Paralogous protein(s)

  • the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity
  • Domains

  • contains four N-acetylglucosamine-polymer-binding LysM domains PubMed
  • C-terminal D,L-endopeptidase domain PubMed
  • Localization

  • cell membrane (according to Swiss-Prot)
  • localizes to cell septa and poles PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon
  • SigH: sigma factor, PubMed, in SigH regulon
  • Regulatory mechanism

  • CcpA: repression, [pubmed|], in CcpA regulon
  • Abh: repression, synergistic transcription repression with AbrB PubMed, in Abh regulon
  • AbrB: repression, synergistic transcription repression with Abh PubMed, in AbrB regulon
  • Regulation

  • repressed by glucose (3-fold) (CcpA) PubMed
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    Biological materials

    Mutant

  • MGNA-B424 (yojL::erm), available at the NBRP B. subtilis, Japan
  • BKE19410 (ΔcwlS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATTCAACCTCCTAAA, downstream forward: _UP4_TAAAAATGAGTATGACAAAA
  • BKK19410 (ΔcwlS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATTCAACCTCCTAAA, downstream forward: _UP4_TAAAAATGAGTATGACAAAA
  • References

    Reviews

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    Original publications

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